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Hepatic UDP-glucose 13C isotopomers from U-13Cglucose: A simple analysis by 13C NMR of urinary menthol glucuronide

机译:来自U-13C葡萄糖的肝UDP-葡萄糖13C同位素异构体:尿薄荷醇葡糖醛酸苷的13C NMR简单分析

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摘要

Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-13C]glucose. Glucuronide 13C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of 13C-13C spin-coupled multiplet components within each 13C resonance by 13C NMR. The [U-13C]glucuronide isotopomer derived via direct pathway conversion of [U-13C]glucose to [U-13C]UDP-glucose was resolved from [1,2,3-13C3]- and [1,2-13C2]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-13C]glucose. In a second study, a group of four overnight-fasted patients (63 ± 10 kg) with severe heart failure were given peppermint oil and infused with [U-13C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-13C]glucose enrichment of 4.6% ± 0.6%. Menthol glucuronide was harvested and glucuronide 13C-isotopomers were analyzed by 13C NMR. [U-13C]glucuronide enrichment was 0.6% ± 0.1%, and the sum of [1,2,3-13C3] and [1,2-13C2]glucuronide enrichments was 0.9% ± 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% ± 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% ± 10% of GP. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc.
机译:摄入400 mg薄荷油和6 g 99%[U-13C]葡萄糖后,从70公斤健康女性受试者的尿液中分离出薄荷醇葡糖醛酸。葡萄糖醛酸化物13C的富集水平为4-6%,因此通过13C NMR为每个13C共振内的13C-13C自旋耦合多重态组分的确定分配提供了高信噪比(SNR)。通过[1,2,3-13C3]-和[1,2-13C2]拆分了通过[U-13C]葡萄糖直接途径转化为[U-13C] UDP-葡萄糖而衍生的[U-13C]葡糖醛酸苷异构体。通过[U-13C]葡萄糖的Cori循环或间接途径代谢得到的葡糖醛酸苷异构体。在第二项研究中,一组四名严重心力衰竭的禁食过夜的患者(63±10千克)被给予薄荷油并注入[U-13C]葡萄糖治疗4个小时(14毫克/千克素乳,0.12毫克/千克/ min恒定输注量),导致稳态血浆[U-13C]葡萄糖富集度为4.6±0.6%。收获薄荷醇葡糖醛酸,并通过13 C NMR分析葡糖醛酸的13 C-异构体。 [U-13C]葡糖苷酸的富集度为0.6%±0.1%,[1,2,3-13C3]和[1,2-13C2]葡糖苷酸的总和为0.9%±0.2%。根据这些数据,血浆葡萄糖向肝UDPG的通量估计为内源性葡萄糖生成(EGP)的15%±4%,而Cori循环至少占GP的32%±10%。 Magn Reson Med,2006年。©2006 Wiley-Liss,Inc.。

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