Application of recombinant techniques for detection of the penaeid shrimp baculovirus, BP

摘要

Research was conducted to develop gene probes to Baculovirus penaei (BP) from a cloning of BP genomic DNA and to test the applications of the gene probes. Five non-homologous probes representing >20% of the total BP genome were tested in dot blot and in situ hybridization assays. Four different dot blot hybridization assay systems were evaluated. Results obtained from radioactive labeling of the probes indicate that the probes are specific for BP DNA, while the results obtained from the three non-radioactive labeling systems suggest problems with non-specific reactivity and sample preparation for these methods. The results also indicated that in situ hybridization is presently the optimum assaying system for BP gene probes. The BP gene probes were used with an in situ hybridization assay to gather initial data concerning the mode of transmission for BP.