Xylanase production by Aspergillus niger ANL 301udusing agro - wastes

摘要

Xylanase production by wild-type Aspergillus niger ANL301, newly isolated from wood-waste, wasudmonitored at 24 h intervals for a period 168 h in media containing different carbon sources. The carbonudsources were oat-spelt xylan (Fluka) and three agro-wastes (sawdust, sugarcane pulp and wheat bran).udHighest xylanase activity of 6.47 units/mL was obtained at 96 h in media containing wheat bran as soleudcarbon source. Maximum activity value for the media containing sugarcane pulp was 0.95 units/mLudobtained also at 96 h. Sawdust and oat spelt xylan gave the peak enzyme activities of 0.65 and 0.80udunits/mL respectively at 120 h. High protein yield was obtained in media containing the agro-wastes,udwith wheat bran giving the highest value of 1.14 mg/mL at 96 h. The maximum specific xylanaseudactivities were 3.86, 3.37, 5.69, and 9.36 units/ mg protein for sawdust, sugarcane pulp, wheat bran andudoat spelt xylan, respectively. Out of the three agro-wastes used in this study, wheat bran holds greatestudpromise for low cost production of the xylanase enzyme.