Extracts of leaves of Eucalyptus camaldulensis and Eucalyptus torelliana were screened phytochemically for the presence of secondary metabolites and for in vitro antibacterial properties. Methanol and dichloromethane extracts of leaves of Eucalyptus camaldulensis and Eucalyptus torelliana were studied for their antibacterial activity against 8 clinically isolated organisms of gastrointestinal origin viz., Klebsiella species UCH 2101, Proteus mirabilis UCH 2102, Proteus mirabilis UCH 2204, Salmonella typhi UCH 2201, Escherichia coli CHO 3101, Escherichia coli UCH 2103, Pseudomonas aeruginosa CHO 3102 and Pseudomonas aeruginosa UCH 2203. The result of the phytochemical screening showed that both extracts contained tannins, saponins, cardiac glycosides but in addition to these, E. torelliana was found to contain anthraquinones. Both extracts were also found to inhibit all the isolates at 10mg mL-1 concentration. The diameter of zones of inhibition exhibited by the extracts was between 10mm and 22mm. The methanol extracts compared favorably with gentamycin used as a standard control. The minimum inhibitory concentrations determined by the agar dilution method were between 0.04mg mL-1 and 10mg mL-1.udThe results obtained from this study reveals that extracts of Eucalyptus camaldulensis and Eucalyptus torelliana possess antibacterial activities against enteric pathogens and the extracts may be a potential source of new antimicrobials against enteric organisms.ududud
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机译:对植物中的次生代谢产物的存在和体外抗菌特性进行了植物化学筛选,从而对camaldulensis桉树和torelliana桉树的叶子提取物进行了植物化学筛选。研究了桉树桉和欧洲桉树叶片的甲醇和二氯甲烷提取物对8种临床分离的胃肠道起源微生物的抗菌活性,这些微生物分别是克雷伯菌属UCH 2101,奇异变形杆菌UCH 2102,变形杆菌Prochus mirabilis UCH 2204,鼠伤寒沙门氏菌U220大肠杆菌CHO 3101,大肠杆菌UCH 2103,铜绿假单胞菌CHO 3102和铜绿假单胞菌UCH2203。植物化学筛选的结果表明,两种提取物均含有单宁,皂角苷,强心苷,但除此以外,还发现了torelliana含有蒽醌。 。还发现两种提取物在10mg mL-1浓度下均能抑制所有分离物。提取物表现出的抑制区域的直径在10mm至22mm之间。甲醇提取物优于庆大霉素作为标准对照。琼脂稀释法测定的最低抑菌浓度为0.04mg mL-1至10mg mL-1。 ud本研究结果表明,桉树桉和ald桉的提取物具有抗肠道病原菌的抗菌活性,提取物可能是新型抗肠道微生物的潜在来源。 ud ud ud
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