Reaksi antigen-antibodi antara protein sub unit pili 18udKDa Shigella flexneri dengan sub unit outer membran protein (OMP) Shigella dysentriae: Strategi memperoleh vaksin shigellosis berbasis protein pili

摘要

Bloody diarrhea disease remains a major cause of the high morbidity and mortality of children globally. Diarrhea caused by S. flexneri and S. dysentriae commonly treated with antibiotics seen already started resistant to existing antibiotics varied while the prevention of shigellosis with Shigella vaccines were developed and used today are still of limited use. This study aims to explore the hemagglutinin protein sub units pili and Omp S. dysentriae suspected having similar characteristics. This study uses Dot Blot and Western Blot to determine the antigen-antibody reaction between the antibody protein sub unit pili S. flexneri with sub unit Omp S. dysentriae. The result showed that the protein sub unit 18 kDa S. flexneri is a protein that is able to agglutinate red blood cells of mice at the highest dilution and is able to recognize sub unit Omp S. dysentriae with a molecular weight of 18 kDa; 21 kDa; and 23 kDa. Protein sub units Omp S. dysentriae with a molecular weight of 18 kDa; 21 kDa; and 23 kDa possibility having epitopes that can be recognized by an antibody protein sub unit pili S. flexneri 18 kDa.