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Isolation and characterization of twelve polymorphic microsatellite loci for the cocoa mirid bug #Sahlbergella Singularis#

机译:可可粉虫#Sahlbergella Singularis#的12个多态微卫星基因座的分离和鉴定

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摘要

Mirids are the primary pests affecting cocoa production in Africa, but no genetic studies have been conducted on these insects. Here we report the isolation and characterization of 12 polymorphic microsatellite loci for Sahlbergella singularis. A microsatellite-enriched genomic DNA library was developed and screened to identify marker loci. Twelve polymorphic loci were identified by screening 28 individuals collected from one presumed population in cocoa plantations in Southern Cameroon. The number of alleles ranged from 5 to 25, whereas the observed and the expected heterozygosities ranged from 0.179 to 0.786 and from 0.671 to 0.946, respectively. Tests showed significant deviations from HW equilibrium for four loci, but no linkage disequilibrium was detected at any of the loci. No cross-species amplification was observed in two other mirid pests in Africa. (Résumé d'auteur)
机译:rid虫是影响非洲可可生产的主要害虫,但尚未对这些昆虫进行遗传学研究。在这里,我们报道了奇异Sahlbergella singularis的12个多态微卫星基因座的分离和表征。开发并富集了微卫星富集的基因组DNA文库,以鉴定标记基因座。通过筛选来自喀麦隆南部可可种植园的一个假定种群的28个人,鉴定了12个多态位点。等位基因的数量范围为5至25,而观察到的和预期的杂合度分别为0.179至0.786和0.671至0.946。测试显示四个基因座与HW平衡存在显着差异,但在任何一个基因座上均未检测到连锁不平衡。在非洲的其他两种线虫中未观察到跨物种扩增。 (Résuméd'auteur)

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