首页> 外文OA文献 >Development of a 63K SNP array for cotton and high-density mapping of intraspecific and interspecific populations of #Gossypium# spp.
【2h】

Development of a 63K SNP array for cotton and high-density mapping of intraspecific and interspecific populations of #Gossypium# spp.

机译:开发用于棉花的63K SNP阵列和#Gossypium#spp种内和种间种群的高密度作图。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

High-throughput genotyping arrays provide a standardized resource for plant breeding communities that are useful for a breadth of applications including high-density genetic mapping, genome-wide association studies (GWAS), genomic selection (GS), complex trait dissection, and studying patterns of genomic diversity among cultivars and wild accessions. We have developed the CottonSNP63K, an Illumina Infinium array containing assays for 45,104 putative intraspecific single nucleotide polymorphism (SNP) markers for use within the cultivated cotton species Gossypium hirsutum L. and 17,954 putative interspecific SNP markers for use with crosses of other cotton species with G. hirsutum. The SNPs on the array were developed from 13 different discovery sets that represent a diverse range of G. hirsutum germplasm and five other species: G. barbadense L., G. tomentosum Nuttal × Seemann, G. mustelinum Miers × Watt, G. armourianum Kearny, and G. longicalyx J.B. Hutchinson and Lee. The array was validated with 1,156 samples to generate cluster positions to facilitate automated analysis of 38,822 polymorphic markers. Two high-density genetic maps containing a total of 22,829 SNPs were generated for two F2 mapping populations, one intraspecific and one interspecific, and 3,533 SNP markers were co-occurring in both maps. The produced intraspecific genetic map is the first saturated map that associates into 26 linkage groups corresponding to the number of cotton chromosomes for a cross between two G. hirsutum lines. The linkage maps were shown to have high levels of collinearity to the JGI G. raimondii Ulbrich reference genome sequence. The CottonSNP63K array, cluster file and associated marker sequences constitute a major new resource for the global cotton research community. (Résumé d'auteur)
机译:高通量基因分型阵列为植物育种社区提供了标准化资源,可用于多种应用,包括高密度遗传作图,全基因组关联研究(GWAS),基因组选择(GS),复杂性状解剖和研究模式品种和野生种之间的基因组多样性。我们已经开发了CottonSNP63K,这是一种Illumina Infinium阵列,其中包含用于栽培棉种Gossypium hirsutum L.中的45,104种推定的种内单核苷酸多态性(SNP)标记的测定方法,以及与其他G品种的杂交所用的17,954种推定的种间SNP标记的测定方法hirsutum。阵列上的SNP由代表不同范围的hirsutum种质和其他五个物种的13种不同发现集开发而成:Barbadense L.,G。tomentosum Nuttal×Seemann,G。mustelinum Miers×Watt,G。armourianum Kearny和G.longicalyx JB Hutchinson和Lee。用1,156个样品验证了阵列,以生成簇位置,以促进对38,822个多态性标记的自动分析。针对两个F2作图群体(一个种内和一个种间)生成了两个包含22829个SNP的高密度遗传图谱,两个图谱中同时出现了3533个SNP标记。产生的种内遗传图谱是第一个饱和图谱,它与26个连锁组相关联,这些连锁组对应于两个G. hirsutum品系之间杂交的棉花染色体数。连锁图谱显示与JGI raimondii Ulbrich参考基因组序列具有较高的共线性。 CottonSNP63K阵列,簇文件和相关的标记序列构成了全球棉花研究界的重要新资源。 (Résuméd'auteur)

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号