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Secondary metabolite localization by autofluorescence in living plant cells

机译:通过自发荧光在活植物细胞中进行次生代谢物定位

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摘要

Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. (Résumé d'auteur)
机译:自发荧光分子在植物细胞中非常丰富,光谱图像为分析其光谱提供了手段,从而产生了有关其积累和功能的信息。基于它们的荧光特性,设计了使用多光子显微镜的成像方法来评估活体植物细胞中内源性荧光团的定位。该方法无需事先处理,可提供一种区分活体组织中多种天然存在的荧光团的有效实验工具。与先进的线性解混技术相结合,光谱分析扩展了可能性,并能够同时检测荧光分子,从而可靠地分离出重叠的发射光谱。但是,与任何技术一样,确实存在人为结果的可能性。这篇方法论文章概述了这些固有荧光团在叶子和果实(此处为咖啡和香草)中组织和细胞内定位的应用。该方法将为研究细胞环境和细胞内环境中内源性荧光团的行为提供新的机会。 (Résuméd'auteur)

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