首页> 外文OA文献 >Multilocus sequence analysis of root nodule isolates from #Lotus arabicus# (Senegal), #Lotus creticus#, #Argyrolobium uniflorum# and #Medicago sativa# (Tunisia) and description of #Ensifer numidicus# sp. nov. and #Ensifer garamanticus# sp. nov.
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Multilocus sequence analysis of root nodule isolates from #Lotus arabicus# (Senegal), #Lotus creticus#, #Argyrolobium uniflorum# and #Medicago sativa# (Tunisia) and description of #Ensifer numidicus# sp. nov. and #Ensifer garamanticus# sp. nov.

机译:#Lotus arabicus#(塞内加尔),#Lotus creticus#,#Argyrolobium uniflorum#和#Medicago sativa#(突尼斯)的根瘤分离株的多基因座序列分析和#Ensifer numidicus#sp。的描述。十一月和#Ensifer garamanticus#sp。十一月

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摘要

Nine isolates from Argyrolobium uniflorum, Lotus creticus, Medicago sativa (Tunisia) and Lotus arabicus (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (recA, atpD, glnA, gltA and thrC), the 16S rRNA gene and the nodulation gene nodA. Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and nodA gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus Ensifer, for which the names Ensifer numidicus sp. nov. (type strain ORS 1407T5LMG 24690T5CIP 109850T) and Ensifer garamanticus sp. nov. (type strain ORS 1400T5LMG 24692T5CIP 109916T) are proposed. The cluster B strains were assigned to Ensifer adhaerens genomovar A. (Résumé d'auteur)
机译:通过多基因座序列分析(MLSA)分析了五个持家基因(recA,atpD,glnA,gltA和thrC),16S rRNA基因和结瘤基因nodA。对单个和串联基因序列的分析表明,这九个新菌株构成了三个稳定的,良好支持的(引导程序和基因序列相似性值)单系簇A,B和C,无论它们属于Ensifer属的分支使用的系统发育重建方法(最大似然,最大简约,邻居加入)。通过API 100辅助造影测试,宿主特异性和nodA基因序列分析进一步表征了这三组。根据这些数据,建议聚类A和C代表Ensifer属中的两个新物种,其名称为Ensifer numidicus sp。十一月(型号ORS 1407T5LMG 24690T5CIP 109850T)和Ensifer garamanticus sp。十一月(型号为ORS 1400T5LMG 24692T5CIP 109916T)。 B株菌株被分配给Ensifer adhaerens genomovar A.(Résuméd'auteur)

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