Purifacation of Wild Type EcoRI Endonuclease and EcoRI Endonuclease RS187Crystal Growth of WT EcoRI Endonuclease-DNA comlex and EcoRI Endonuclease

摘要

EcoRI endonuclease is a very useful tool to study the structural mechanism of protein-DNA recognition. In this work, wild type EcoRI Endonuclease and EcoRI Endonuclease mutant RS187 were purified to high purity. Crystals of wild type EcoRI Endonuclease-DNA 13mer complex have been obtained with good size and shape. Some small crystals of EcoRI Endonuclease RS187-DNA 13mer complex were also grown.