How bacteria reproduction differs on the surface of different types of fishes; Sparus aurata, Dicentrarchus labrax, Merlangius merlangus, Engraulis encrasicolus kept in refrigerator for four days at 4°C, indicated by counting the number of colonies reproduced on eosin methylene-blue lactose sucrose agar plate in 24 hours at 37°C under controlled conditions (temperature, light intensity, moisture etc.) in a culture incubator?

摘要

Fishes are considered as one of the healthiest foods because of omega-3, protein and vitamins that they contain. However, food poisoning from fish is seen commonly. Inadequate storage conditions are the main reason and bacteria growth that cause illness differs from species to species. The aim of this research is to observe, compare and contrast the bacterial growth on the surface of four different fish species after four days of storage duration in refrigerator.udThe research question of this investigation is: “How bacteria reproduction differs on the surface of different types of fishes; Sparus aurata, Dicentrarchus labrax, Merlangius merlangus, Engraulis encrasicolus kept in refrigerator for four days at 4°C, indicated by counting the number of colonies reproduced on eosin methylene-blue lactose sucrose agar plate in 24 hours at 37°C under controlled conditions (temperature, light intensity, moisture etc.) in a culture incubator?”udThe method used in this experiment is viable cell counting method. The fishes are bought freshly from the fish market and kept in the refrigerator for four days in stretch wrapped plates at 4°C. A sample is taken from each fish surface, incubation of the sample is done on EMB agar plates for 24 hours at 37°C. Bacterial colonies are counted after 24 hours.udThe mean results of living bacteria colony number are as follows: 50 on Merlangius merlangus, 98,4 on Dicentrarchus labrax, 153,6 on Engraulis encrasicolus , and 198,6 on Sparus aurata. These results showed that on Sparus aurata the bacteria reproduction was the highest. The least bacteria reproduction was observed on Merlangius merlangus. According to this result, Merlangius merlangus is the best one for storage at 4°C. The conclusion is that bacteria reproduces on each four species of fishes and not edible after such duration. Anova calculation showed that p value is 3,59197x〖10〗^(-21), which indicates there is a meaningful statistical difference in bacterial colony reproduction on the surface of different fish types.