Thg-1pit gene expression in granule cells of the developing mouse brain and in their synaptic targets, mature Purkinje and mitral cells.

摘要

We have studied the expression of Thg-1pit in developing and adult mouse brain by in situ hybridizationanalysis. We show that, at day 12.5 of embryo development, Thg-1pit expression is restricted to the rhombiclip, subventricular neuroepithelium/mantle zone, and lateral ganglionic eminence, namely the embryonicbrain areas where granule cell precursors originate. Thereafter, Thg-1pit expression landmarks bothdifferentiative steps and the mature function of granule/interneuron cells in several brain districts,including cerebellum, basal forebrain, olfactory bulb, and hippocampus. In the adult, Thg-1pit becomes alsoactivated in mitral cells of olfactory bulb and in Purkinje cells of cerebellum, in concomitance with fulldevelopment of the synaptic contacts that Purkinje and mitral cells establish with granule cells. Weconclude that Thg-1pit is relevant to specification, proliferation/migration, differentiation, and maturefunction of granule/interneuron cells in different brain districts, as well as to the function of mature, but notimmature, Purkinje cells and mitral cells