DNA Biochips for Microbial Pathogen Detection Based on Fluorescence or CMOS Process

摘要

Number of pathogenic tick-borne bacteria cause diseases in humans andudanimals. Wide range of conventional methods as PCR, immuno-based detection,udcultivation, reverse line blot or microscopy is available for identification of bacterialudpathogens. Although, these methods are used often in laboratories, they do not allowudsimultaneous detection of wide spectrum of bacteria. DNA chips represent technologyudfor fast, sensitive, relatively cheap and simultaneous detection of microorganisms inudbiological samples.udWe have developed the oligo-chip for detection of different tick-borne pathogens.udThis method is based on detection of fluorescent signal after hybridization reaction byudusing laser scanner. We are also working on preparation of electronic device foruddetection of specific contact of two single complementary strands of DNA. This methodudwill allow simple detection without using the fluorescent probes and expensive laserudscanner. For this purpose a novel solid-state sensor for detection of bio-molecularudprocesses was developed. The device is compatible with a standard CMOS process,udproviding fully electronic readout and large-scale of integration of biosensors on a singleudchip. A model of the device was developed and simulated.