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Microcystin-LR biodegradation by Sphingopyxis sp USTB-05

机译:鞘氨醇单胞菌USTB-05对微囊藻毒素LR的降解

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摘要

A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as Sphingopyxis sp. USTB-05, which was the first isolated MCs-biodegrading Sphingopyxis sp. in China. The average biodegradation rate of MC-LR by Sphingopyxis sp. USTB-05 was 28.8 mg center dot L(-1) per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30A degrees C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg center dot L(-1) in water was biodegraded by Sphingopyxis sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg center dot L(-1) could be completely removed in 3 h by CE of Sphingopyxis sp. USTB-05 containing 350 mg center dot L(-1) protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.
机译:从中国滇池成功分离出一种有前途的细菌降解微囊藻毒素-LR(MC-LR)作为唯一的碳和氮源的菌株。该菌株被鉴定为Sphingopyxis sp.。 USTB-05是第一个分离的可降解MCs的Sphingopyxis sp.。在中国。 Sphingopyxis sp。的MC-LR的平均生物降解率。 USTB-05每天为28.8 mg中心点L(-1),显然高于迄今为止报道的其他细菌的水平。菌株USTB-05生长和MC-LR生物降解的最佳温度和pH分别为30A摄氏度和7.0。研究了从贵水湖收集的蓝细菌细胞中MC-LR的释放以及菌株和无细胞提取物(CE)对MC-LR的生物降解。结果表明,Sphingopyxis sp。降解了初始浓度为4.0 mg中心点L(-1)的MC-LR。 USTB-05在4天内,而最初浓度为28.8 mg中心点L(-1)的MC-LR可以通过Sphingopyxis sp的CE在3小时内完全去除。 USTB-05包含350 mg中心点L(-1)蛋白。在酶促降解MC-LR的过程中,在HPLC色谱图上观察到两个中间代谢物和一个死端产物。此外,MC-LR及其代谢产物的相似扫描图谱表明,MC-LR的Adda侧链在所有产物中均保持完整。

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