高速液体クロマトグラフィーによるセ口オリゴ糖の簡便な調製法

摘要

A convenient isolation method of cello-oligosaccharides was presented. The cello-oligosaccharide mixture was prepared by partial hydrolysis of Whatman CF-11 cellulose powder with 72％ sulfuric acid for 1 hr at 5℃ followcd by extraction with 1 : 1 (v/v) mixture of acetonitrile and water. It was found that the high performance liquid chromatography on a new silica gel column, chemically modified with polyamine resin, was very effective not only for analytical point of view but also for isolation of cello-oligosaccharides up to degree of polymerization of 8