A Study of the Polarographic Quantitative Analysis of the Urinary 17-Ketosteroids II. Fractionation of the Urinary Neutral 17-Ketosteroids by Means of Polarographic Estimation and Chromatographic Pattern of the Urinary Neutral 17-Ketosteroids in Healthy Man

摘要

There has been no report available at present for app l i cation of polograph to chromatographic fractionation of urinary neutral 17-ketosteroids. In 1940 Wolfe described that the same volumes of androsterone, isoandrosterone, and dehydroisoandrosterone produced the same polarogram even if their molecular weights were not identical. Both Wolfe's and Wolfe-Kimoto's methods were not satisfactory for applying them to chromatographic fractionation so that the author intented to improve polarographic analysis as mentioned in Part I. And chromatographic patterns of urinary neutral 17-ketosteroids in 11 healthy adult males were produced by application of polarograph to microscale liquid column partition chromatography (Edwards-Ohno-Wakoh). In colorimetric quantitative analysis o f urinary 17-ketosteroids, 150-200c c of urine in daily ouyput, equivalent to 500-600y of 17- k etosteroids contained, is employed, while in polarographic analysis 300-40c0c of urine, equivalent to 1 mg of 17-ketosteroids, was employed by the author. In a case of 32 years old male, polarogram consisted of 8 fractions each of which possesses a peak was presented and the ratio of IV-I-V to VI-EVII was more than 1.75 and furthermore IV was higher than V. It is worth to note that I and VIII fractions showed relatively lower value comparing with colorimetric analysis.