THE DRUG SENSITIVITY OF VP-16 IN VITRO IN BLADDER CANCER CELL LINES,KK-47,KW-103, AND RT 4 AND IN RENAL CANCER CELL LINE, KN-41

摘要

Using a colony formation technique and KK-47, KW-103 and RT4 cell lines established from human bladder transitional cell carcinomas and KN-41 cell line from a human renal cell carcinoma, the drug sensitivity of VP-16 was determined. The survival curves of the cell lines showed that this agent has a concentration dependent cell killing effect. From the drug sensitivity at 50 per cent and 90 per cent inhibitions (ID50 and ID90) determined from the survival curves in these cell lines at 2-and 24-hour exposure series of the drug, it was indicated that VP-16 has almost the same cell killing effects as those of bleomycin and cis-platinum. Compared with those of mitomycin C, adriamycin and carboquone, the ID50 and IDgo values of VP-16 were I to 3 digits higher in order.