Studies on male infertility. Lactic dehydrogenase isozymes of the seminal plasma in infertile men

摘要

1. LDH-isozymes of the seminal plasma was investigated in 171 infertile men. Polyacrylamide gel disc electrophoresis was used to obtain zymograms. In 63 cases, concentration of each isozyme fraction was determined with microdensitometer. 2. Out of 34 azoospermic cases, 26 seminal plasmas showed only three bands of LDHisozymes. They consisted of postvasectomy 3, germ cell apJasia 10, spermatogenic arrest 8, and hypospermatogenesis 5. 3. In 106 oligozoospermic cases, number of LDH-isozyme bands was correlated with sperm concentration. 4. No correlation was found between sperm motility rate and number of LDH-isozyme bands. 5. Densitometry of LDH-isozyme bands demonstrated constant ratio of them without regard to histological findings of the testes. On the other hand, oligozoospermic and normozoospermic cases showed high concentration of LDH3 in the three-bands group or of LDH5 in the five-bands group in good correlation with sperm concentration.