Fluorescent in situ hybridization and flow cytometryudas tools to evaluate the treatments for the controludof slime-forming enterobacteria in paper mills

摘要

Slime formation is a serious problem nowadaysudin the paper industry. Some enterobacteria are associatedudwith the formation of slime deposits in paper and boardudmills. Detection and characterization of slime formingudbacteria, belonging to the genus Enterobacter, Raoultella,udand Klebsiella have been achieved by fluorescence in situudhybridization (FISH), using one probe based on theudenterobacterial repetitive intergenic consensus sequenceudand other two rRNA targeted oligonucleotide probes. Theudeffects of three kinds of antimicrobiological productsud(biocides, dispersants, and enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming enterobacteria and to select specific treatments to control microbial problems in the paper industry.