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Large scale purification of presynaptic plasma membranes from Torpedo marmorata electric organ

机译:mar鱼雷电器官中突触前质膜的大规模纯化

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摘要

The presynaptic plasma membrane (PSPM) of cholinergic nerve terminals was purified from Torpedo electric organ using a large-scale procedure. Up to 500 g of frozen electric organ were fractioned in a single run, leading to the isolation of greater than 100 mg of PSPM proteins. The purity of the fraction is similar to that of the synaptosomal plasma membrane obtained after subfractionation of Torpedo synaptosomes as judged by its membrane-bound acetylcholinesterase activity, the number of Glycera convoluta neurotoxin binding sites, and the binding of two monoclonal antibodies directed against PSPM. The specificity of these antibodies for the PSPM is demonstrated by immunofluorescence microscopy.
机译:胆碱能神经末梢的突触前质膜(PSPM)使用大规模程序从鱼雷电器官中纯化。一次可分离多达500 g的冷冻电器官,从而分离出大于100 mg的PSPM蛋白。根据其膜结合的乙酰胆碱酯酶活性,回旋甘油神经毒素结合位点的数量以及两种针对PSPM的单克隆抗体的结合判断,该级分的纯度类似于在鱼雷突触体细分后获得的突触质膜的纯度。这些抗体对PSPM的特异性已通过免疫荧光显微镜证实。

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