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Biochemical composition and bioactivity screening of various extracts from dunaliella salina, a green microalga

机译:一种绿色微藻盐藻盐藻的各种提取物的生化组成和生物活性筛选

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摘要

The current study examines the antimicrobial and antioxidant properties of different extracts of the microalgaDunaliella salina Teodoresco (Dunaliellaceae), their fatty acid composition and the antimicrobial activity of the oil. Antimicrobial and antioxidant activities were evaluated by obtaining extracts of D. salina in n-hexane, dichloromethane, ethanol, and methanol. To evaluate the antimicrobial activity, the extracts, and fatty acids from D. salina were assessed by the disc diffusion and microdilution techniques against pathogenic microorganisms including fish and clinical/food-borne. The MBC or MFC values of the extracts and fatty acids ranged from 0.63 to 10.00 mg/ml. The antioxidant activity was studied by phosphomolybdenum and DPPH assays and β-carotene/linoleic acid tests. In addition, the total phenolic and flavonoid contents were evaluated and the fatty acid composition was determined using gas chromatography. Palmitic, alpha-linolenic, and oleic acids were discovered to be the major components of the fatty acids. These findings have demonstrated that the extracts and oil from D. salina could be used as natural antimicrobials and antioxidants in the food/feed and pharmaceutical industry and as a biodiesel because of its high unsaturated fatty acid content.
机译:当前的研究检查了微藻藻杜氏盐藻(Dunaliellaceae)(杜氏藻科)的不同提取物的抗微生物和抗氧化性能,其脂肪酸组成和该油的抗微生物活性。通过获得在正己烷,二氯甲烷,乙醇和甲醇中的盐藻D. salina的提取物来评估其抗菌和抗氧化活性。为了评估抗菌活性,通过圆盘扩散和微量稀释技术对来自鱼类(D. salina)的提取物和脂肪酸进行了评估,以针对包括鱼类和临床/食源性在内的病原微生物。提取物和脂肪酸的MBC或MFC值范围为0.63至10.00 mg / ml。通过磷钼和DPPH分析以及β-胡萝卜素/亚油酸试验研究了抗氧化活性。另外,评估了总酚和类黄酮含量,并使用气相色谱法测定了脂肪酸组成。发现棕榈酸,α-亚麻酸和油酸是脂肪酸的主要成分。这些发现表明,来自盐生杜鹃的提取物和油可在食品/饲料和制药行业中用作天然抗菌剂和抗氧化剂,并由于其不饱和脂肪酸含量高而可用作生物柴油。

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