Development and characterisation of novel microsatellite loci for the baldchin groper (Choerodon rubescens) and cross-amplification in seven other labrid species

摘要

We describe the development and characterisation of novel microsatellite loci for the baldchin groper, Choerodon rubescens. The purpose was to identify loci that can be used to resolve uncertainties about the population (stock) structure of this fish species, which is endemic to a narrow region of the west coast of Australia and showing evidence of overfishing in some locations. Of 22 loci characterised, 12 appear to be ideally suited for population-level analyses. Utilising data obtained from four sampling locations across the distribution of C. rubescens, the total number of alleles observed at each of the 12 loci ranged from 2 to 24, while the overall values of expected heterozygosity ranged from 0.19 to 0.89. Cross-amplification of the 12 loci in seven other labrid species was often successful, especially in congeners.