Advanced wheat lines carrying the Hessian fly resistance gene H27 were obtained by backcrossing the wheat/Aegilops ventricosa introgression line, H-93-33, to commercial wheat cultivars as recurrent parents. The Acph-N v 1 marker linked to the gene H27 on the 4Nv chromosome of this line was used for marker assisted selection. Advanced lines were evaluated for Hessian fly resistance in field and growth chamber tests, and for other agronomic traits during several crop seasons at different localities of Spain. The hessian fly resistance levels of lines carrying the 4Nv chromosome introgression (4D/4Nv substitution and recombination lines that previously were classified by in situ hybridisation) were high, but always lower than that of their Ae. ventricosa progenitor. Introgression lines had higher grain yields in infested field trials than those without the 4Nv chromosome and their susceptible parents, but lower grain yields under high yield potential conditions. The 4Nv introgression was also associated with later heading, and lower tiller and grain numbers/m2 . In addition, it was associated with longer and more lax spikes, and higher values of grain weight and grain protein content. However, the glutenin and gliadin expression, as well as the bread-making performance, were similar to those of their recurrent parents
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机译:携带黑森州抗蝇基因H27的高级小麦品系是通过将小麦/埃及埃菲洛斯甜菜渗入系H-93-33回交给商业小麦品种作为轮回亲本而获得的。与该品系4Nv染色体上的基因H27连接的Acph-N v 1标记用于标记辅助选择。在田间和生长室测试中对先进的品系进行了黑森州抗性的评估,并在西班牙不同地区的几个作物季节中评估了其他农艺性状。携带4Nv染色体基因渗入的品系(以前通过原位杂交分类的4D / 4Nv替代和重组品系)的黑森州蝇抗性水平很高,但始终低于其Ae。心室祖细胞。与没有4Nv染色体及其易感亲本的玉米相比,在侵染的田间试验中,渗入系的籽粒产量更高,但在高产潜力条件下,籽粒的产量较低。 4Nv渗入也与后期抽穗,较低的分till和籽粒数/ m2有关。另外,它与更长和更多的松散穗以及更高的谷物重量和谷物蛋白含量有关。然而,谷蛋白和麦醇溶蛋白的表达,以及面包的生产性能,与他们的亲生父母相似。
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