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Mapping quantitative trait loci (QTLs) associated with dough quality in a soft × hard bread wheat progeny

机译:绘制与软×硬面包小麦后代中面团质量相关的定量性状位点(QTL)

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摘要

Bread wheat (Triticum aestivum L.) quality is a key trait for baking industry exigencies and broad consumer preferences. The main goal of this study was to undertake quantitative trait loci (QTL) analyses for bread wheat quality in a set of 79 recombinant inbred lines (RILs) derived from a soft × hard bread wheat cross. Field trials were conducted over two years, utilizing a randomized complete block design. Dough quality was evaluated by sedimentation test, mixograph and alveograph analysis. Protein content was measured by near-infrared reflectance analysis and grain hardness was determined by the single kernel characterization system (SKCS).udA genetic map based on 263 SSR markers and glutenin loci was constructed. Composite interval mapping (CIM) analysis detected a total of 20 QTLs distributed among ten chromosomes which were associated with variations in quality traits.udResults confirmed the previous investigations on the known relationship between storage-protein alleles and dough quality, and detected new and stable QTLs related to dough quality parameters on chromosomes 2A, 7A, 5B and 1D. These new QTLs could be further investigated. Also, in this study, some RILs showed very high dough extensibility values which involve future validation studies for QTLs associated with to this trait.
机译:面包小麦(Triticum aestivum L.)的品质是烘焙行业的迫切需求和广泛的消费者偏爱的关键特征。这项研究的主要目的是对来自软×硬面包小麦杂交的79个重组自交系(RIL)进行面包小麦品质的定量性状基因座(QTL)分析。利用随机完整模块设计进行了两年的现场试验。面团质量通过沉降测试,混合仪和水泡仪分析进行评估。通过近红外反射率分析测量蛋白质含量,并通过单核表征系统(SKCS)确定谷物硬度。 ud基于263个SSR标记和谷蛋白位点构建了遗传图谱。复合间隔图(CIM)分析检测到总共20个QTL,分布在10条染色体中,这些QTL与品质性状的变化有关。与2A,7A,5B和1D染色体上的面团质量参数有关的QTL。这些新的QTL有待进一步调查。同样,在这项研究中,一些RIL显示出很高的面团延展性值,涉及与该性状相关的QTL的未来验证研究。

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