Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms

摘要

The characteristics of the inoculum are recognized as one of the most relevant factors influencing the results of in vitro fermentations in batch cultures of ruminal microorganisms. Four rumen-fistulated sheep fed a 66:34 alfalfa hay:concentrate diet were used as donors to investigate the effect of rumen contents’ processing on in vitro methane (CH4) and volatile fatty acid (VFA) production from 3 substrates of variable composition.