Rediscovery of historical vitis vinifera varieties from South Anatolia region by using amplified fragment lenght polymorphism and simple sequence repeat dna fingerprinting methods

摘要

Anatolia is thought to have played an important role in the diversification and spread of economically important crop plants including Vitis vinifera. Although, various biodiversity studies have been already conducted with local grape varieties in different regions of Anatolia, our aim is to contribute to gain better knowledge on the biodiversity of historical grape varieties in North, Adana region of South Anatolia. In the present study, microsatellites were used for pre-selection of 27 collection varieties among 72 grape accessions obtained from National Germplasm Vineyards, for further determination of genetic relatedness among studied grape accessions amplified fragment length polymorphims (AFLPs) were utilized. The unweighted pair group method with arithmatic mean (UPMGA) cluster and principal component analyses revealed that Saimbeyli accessions form a distict group which are distantly related to collection varieties from all over Turkey. The results of this study revealed the genetic similarities of some isolated historical Anatolian grape varieties cultivated for decades in local vineyards in Saimbeyli, Adana. To our knowledge, this is the first study conducted with these varieties. Further preservation and use of these varieties will be helpful to avoid genetic erosion and diversity loss in this part of Anatolia.