Background: Mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour, antiviral and antibacterial activities. The present study involved the production, purification and characterisation of intracellular and extracellular free and protein-bound polysaccharides from Pleurotus ostreatus and the investigation of their growth-inhibitory effect on human carcinoma cell lines. Results: Several fermentation parameters were obtained: batch polysaccharide productivities of 0.013 +/- 8.12 x 10-5 and 0.037 +/- 0.0005 g L-1 day-1 for intracellular and extracellular polysaccharides respectively, a maximum biomass concentration of 9.35 +/- 0.18 g L-1, Pmax = 0.935 +/- 0.018 g L-1 day-1, µmax = 0.218 +/- 0.02 day-1, YEP/X = 0.040 +/- 0.0015 g g-1 and YIP/X = 0.014 +/- 0.0003 g g-1. Some polysaccharides exhibited superoxide dismutase (SOD)-like activity of 50-200 units. Fourier transform infrared analysis of the polysaccharides revealed absorption bands characteristic of such biological macromolecules. Cytotoxicity assays showed that both intracellular and extracellular polysaccharides exhibited antitumour activity towards several tested human carcinoma cell lines in a dose-dependent manner. Conclusion - The polysaccharides of P. ostreatus exhibited high SOD-like activity, which strongly supports their biological effect on tumour cell lines. The extracellular polysaccharides presented the highest antitumour activity towards the RL95 carcinoma cell line and should be further investigated as an antitumour agent.
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机译:背景:蘑菇多糖在功能性食品中起重要作用,因为它们具有生物调节特性,例如抗肿瘤,抗病毒和抗菌活性。本研究涉及平菇的胞内和胞外游离和蛋白结合多糖的生产,纯化和表征,以及它们对人癌细胞系生长抑制作用的研究。结果:获得了多个发酵参数:细胞内和细胞外多糖的批量多糖生产率分别为0.013 +/- 8.12 x 10-5和0.037 +/- 0.0005 g L-1 day-1,最大生物质浓度为9.35 +/- 0.18 g L-1,Pmax = 0.935 +/- 0.018 g L-1 day-1,µmax = 0.218 +/- 0.02 day-1,YEP / X = 0.040 +/- 0.0015 g g-1和YIP / X = 0.014 +/- 0.0003克g-1。一些多糖表现出50-200个单位的超氧化物歧化酶(SOD)样活性。多糖的傅立叶变换红外分析揭示了这种生物大分子的吸收带。细胞毒性测定表明,细胞内和细胞外多糖均以剂量依赖的方式对几种测试的人类癌细胞系表现出抗肿瘤活性。结论-平菇多糖具有较高的SOD样活性,强烈支持其对肿瘤细胞系的生物学作用。细胞外多糖对RL95癌细胞系具有最高的抗肿瘤活性,应进一步研究其作为抗肿瘤剂。
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