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Enterobacter pulveris, sp. nov. isolated from fruit powder, infant formula and infant formula production environment.

机译:粉状肠杆菌十一月从水果粉,婴儿配方食品和婴儿配方食品的生产环境中分离出来。

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摘要

Six Gram-negative, facultative anaerobic, non-spore-forming isolates of coccoid rods were obtained from fruit powder (n=3), infant formula (n=2) and infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB sequence analysis, allocated the isolates to the Enterobacteriaceae. The highest rpoB sequence similarities (91.2-95.8 %) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species is supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the positive utilization of α-D-melibiose, sucrose, D-arabitol, mucate and 1-0-methyl-α-galacto-pyranoside as well as negative tests for D-sorbitol, and the Voges-Proskauer reaction. \udOn the basis of the phylogenetic analyses, DNA-DNA hybridizations and the unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel Enterobacter species, Enterobacter pulveris sp. nov. The type strain is 601/05T (= LMG 24057T = DSM 19144T).
机译:从水果粉(n = 3),婴儿配方食品(n = 2)和婴儿配方食品生产环境(n = 1)中获得了六种革兰氏阴性,兼性厌氧,非孢子形成的球状棒状分离物,并在多相中进行了研究分类学研究。比较16S rRNA基因序列分析与rpoB序列分析相结合,将分离株分配到肠杆菌科。与瑞士肠杆菌,放射线肠杆菌,土产肠杆菌和阪崎肠杆菌获得最高的rpoB序列相似性(91.2-95.8%),并且由这些物种形成的系统发育分支具有较高的自举值。生化数据显示,可以通过积极利用α-D-杂糖,蔗糖,D-阿拉伯糖醇,粘液和1-0-甲基-α-半乳糖吡喃糖苷以及D的阴性试验,将分离株与最近的邻居区分开。 -山梨糖醇和Voges-Proskauer反应。根据系统发育分析,DNA-DNA杂交以及独特的生理和生化特征,提出了分离株代表一种新型肠杆菌种,即粉状肠杆菌。十一月类型应变为601 / 05T(= LMG 24057T = DSM 19144T)。

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