Preparation and characterization of sodium poly(aL-glutamate)/poly(pyrrole)/poly(ethylene oxide) polymeric blends for a responsive peptide drug delivery system

摘要

Blends of sodium poly(a,L-glutamate) (MW 49K), poly(pyrrole) and poly(ethylene oxide) (MW lOOK) were prepared by a solution-blending process. A series of blend compositions were studied and characterized to determine optimal parameters and conditions necessary for the encapsulation and stability of the peptide portion of this ternary system. The retaining of the conformational structure of the peptide is important for its activity in a delivery system. Results derived from Infrared Spectroscopy (lR) indicated that the helical conformation of the peptide was maintained. The solid-state morphological properties of the ternary blends were studied by Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). Results suggest the formation of a solid-state system with miscible properties that are due to noncovalent interactions, such as, ion-dipole and hydrophobic interactions. Preliminary cell culture studies utilizing ovarian cancer cells indicate that these cells will proliferate and adhere to the surface of these polymer blends.