In-vitro bioassays of culturefiltrate of endophytic fungi Trichoderma sp. In different medium against root-Knot Nematode Meloidogyne spp.

摘要

Trichoderma sp. is one of the endophytic fungi, which is produce toxic culturefiltrate against plant pathogen e.g plant parasitic nematode. Experiments were conducted to in vitro bioassays of the antagonistic effects of culturefiltrate produced by endophytic fungi Trichoderma sp. in different medium on mobility and mortality of Meloidogyne-J2. Three different medium of culturfiltrate were investigated for the in vitro bioassays against Meloidogyne-J2. Inactivation/paralysis and mortality were recorded at 1, 3 and 24 hours of exposure to culturefiltrates. All of treatment of three different medium were statistically different on inactivation/paralysis of root-knot nematode Meloidogyne-J2 to compare with control (H2O and H2O + Trichoderma sp). Mortality of root-knot nematode Meloidogyne-J2 after 24 hrs exposed to culturefiltrate, followed by rinsing with sterile distilled water, demonstrated that the effects of culturefiltrates of the treatment of Czapeks-Dox + Trichoderma sp and GFM + Trichoderma sp were irreversible, as nematodes did not recover and were considered dead.