Potential use of cholinesterase activity from tropical green mussel, Perna viridis as a biomarker in effect-based marine monitoring in Indonesia

摘要

Green mussels (Perna viridis) play an important economic and ecological role in the coastal areas of Indonesia. P. viridisudhas been used as an eco-sentinel organism for marine biomonitoring program in Asia regions. To magnify its competency as audrelevant organism in biomonitoring, the cholinesterases of P. viridis from a selected coastal area of Indonesia have been characterized.udDissected organs which were gill, foot, mantle and posterior adductor muscle were examined for substrate specificityudand inhibitors sensitivity using selective and non-selective substrates and inhibitors. The highest level of cholinesterase activityudwas observed in gill and followed by foot, mantle and posterior adductor muscle. The substrate specificity approach using variousudalkylthiocholines indicated that the cholinesterases of P. viridis constitute an acetylcholinesterase (AChE) and a butyrylcholinesteraseud(BuChE). Likewise, the inhibitors sensitivity approach using eserine, BW284C51, and iso-OMPA sustained theudprevious approach which recorded typical AChE and atypical BuChE. The application of cholinesterase activity in three selectedudareas of Indonesian waters suggested that the gill of P. viridis is the most suitable organ for employing the ChE activity as a biomarkerudin effect-based marine monitoring.