PRODUCTION AND CHARACTERIZATION OF CHITINASE ENZYMES FROM SULILI HOT SPRING IN SOUTH SULAWESI, Bacillus sp. HSA,3-1a

摘要

Chitinase is an extracellular enzyme which is capable in hydrolyzing insoluble chitin to its oligomeric andudmonomeric components. The enzyme produced by thermophilic bacteria was screened and isolated from Sulili hotudspring in Pinrang, South Sulawesi, Indonesia. The gram positive spore forming rod shape bacteria was identified asudBacillus sp. HSA,3-1a through morphological and physiological analysis. The production of chitinase enzyme wasudconducted at various concentration of colloidal chitin at a pH of 7.0 and a temperature of 55 ??C. The bacteriaudoptimally was produced the enzyme at a colloidal chitin concentration of 0.5% after 72 h of incubation. The optimumudtemperature, pH and substrate concentration of chitinase were 60 ??C, 7.0 and 0.3%, respectively. The enzyme wasudstable at a pH of 7.0 and a temperature of 60 ??C after 2 h of incubation. The chitinase activities was increased byudaddition of 1 mM Mg2+, Ca2+ and Mn2+ ions, whereas the activities were decreased by 1 mM Co2+, Fe2+ and Zn2 ions.udThe molecular weight of the crude enzyme was determined using SDS-PAGE analysis. Five protein fractions wereudobtained from SDS-PAGE, with MWs of 79, 71, 48, 43 and 22 kDa