Acid mine drainage (AMD) was the waste from the mine of industry, could be overcome used by microbial biology. This research has aimed to determine the effect of marsh sediments in reduced the content of sulfate and increased pH in acid mine drainage, known the growth of microbial populations in the treatment of acid mine water marsh sediments, and for known the kind of microbes based on morphological characteristics. AMD treatment was made with three kinds of treatment were treatment I was AMD with the addition of marsh sediments and compost, treatment II with the addition of marsh sediments and treatment III, AMD without the addition of marsh sediments and compost as control. Measurement of sulfate content by titration method, measure of pH by used a pH meter,and calculation of the total microbial by Standard Plate Count (SPC) method. Results of research showed that microbes derived from marsh sediments were added compost as a carbon source could decreased AMD sulfate from 529,00 ppm on day 0 into 147,64 ppm on day 25. AMD treatment with marsh sediments without the addition of compost, sulfate also decreased from 529,00 ppminto 277,10. While the AMD treatment as a control,did not change the levels of sulfate. Measure pH in the treatment AMD with the addition of marsh sediment and compost increased pH from 2,967 on day 0 into 7,739 on day 25. AMD treatment with marsh sediments without the addition of compost, pH also increased from 2,967 into 6,554. While the AMD treatment as a control, pH AMD until until day 25 stillindicates acid conditions. The total number of microbes in treatment I and II treatment increased microbial growth. Results of the microbial isolation from marsh sediments, obtained 13 different microbial isolates based morphology.
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