IMPLICATION OF CLAY MODIFIED ELECTRODE ON CYANIDE BIOSENSOR

摘要

A novel, inexpensive and simple amperometric biosensor based on immobilization of udtyrosinase enzyme onto bentonite and modified bentonite is applied for determination of udcyanide. The determination of cyanide was performed via its inhibiting action on the udtyrosinase electrode. Measurement was carried out using cathecol as substrate. The udenzymatically generated quinoid products were electroreduced at 0.1 V vs Ag/AgCl. An udextremely sensitive detection limit (2 x 10-7 M) was obtained for cyanide. Enzyme udimmobilitation onto an modified bentonite seems to cause an increase in cyanide inhibition because of the greater surface area.