The use of cholinergic biomarker, cholinesterase activity of blue mussel Mytilus edulis to detect the effects of organophosphorous pesticides

摘要

The aim of the study was to investigate the effects of organophosphorous pesticide on the cholinesteraseudactivity of different organs of Mytilus edulis. The mussels were exposed to serial dilutions of theudpesticides (0, 50, 100, 200, 500, and 1000 ??g/l) for 96 h. A significant inhibition of the cholinesteraseudactivity from gill occurred at the lowest concentration, which indicated that gill was the most sensitiveudorgan. The moderate sensitive organs were foot and mantle, which were inhibited by trichlorfon at 200ud??g/l. The least sensitive organs were hemolymph, posterior adductor muscle and digestive gland wereudinhibited at 1000 ??g/l. After incubation of the trichlorfon-exposed mussels for seven days in clean media,udthe cholinesterase activities from different organs of mussels were not cured completely. Theudcholinesterase activities from hemolymph, gill, posterior adductor muscle and digestive gland recovered,udwhile the persistent inhibition of cholinesterase activity from foot and mantle were observed.