Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production

摘要

Microalgae is one of potential source for biodiesel due to high efficiency of solar energy conversion to chemical energy.udSeveral microalgae also have high lipid content per dry weight of biomass. The aims of the present work to study the effects ofudtemperature and nutrient depletion on the growth and lipid content of three selected microalgae (Dunaliella tertiolecta,udNannochloropsis sp and Scenedesmus sp) in view of their possible utilization as raw materials for biodiesel production. In addition,udvarious lipid analysis methods were applied, such as gravimetric, Nile Red staining and FTIR spectroscopy. Algal growth and lipidudcontent was strongly influenced by the variation of tested parameters; indeed, an increase or decrease temperature from ambientudtemperature and nutrient depletion practically increase in lipid content. Nile Red staining and FTIR spectroscopy are effective tool toudanalyze rapidly of lipid content from selected microalgae.