Fabrication of electrochemical sensor modified with nano graphene for determination of warfarin in plasma samples

摘要

Background and aims: Warfarin is anticoagulant drug and prevents thromboembolism in cases such as pulmonary embolism and blood clot in a leg vein which under high risk of thrombosis. The therapeutic window of warfarin is very narrow. Therefore, it is important to monitoring the level of warfarin in blood patient. Electrochemical sensors are powerful tools in the field of diseases diagnosis and medical care, due to advantages such as high selectivity, high sensitivity and low. The aim of this study was fabrication of an electrochemical sensor base on modification of carbon electrode using graphene nanoparticle for quantification of warfarin in plasma.udMethods: In this study, plasma sample of 8 patients who had consumed warfarin were analyzed. After precipitation of plasma proteins by acetonitrile, the sample was centrifuged and the supernatant was transferred to a test tube. The resulting solution was evaporated by stream of nitrogen gas to complete drying. The dry residue was diluted by distilled water and transferred into the voltammetric cell for evaluation of warfarin oxidation by sensor.udResults: The results show that fabricated nano sensor strongly catalyzes the oxidation current of warfarin. Furthermore there are linear relationship (least squares method) between oxidation current of warfarin and its concentration in plasma.udConclusion: The sensor as a simple, low-cost and accurate procedure is able to measure warfarin in patients' plasma.