Preparation and antiherpetic activities of chemically modified polysaccharides from Polygonatum cyrtonema Hua

摘要

The chemically modified polysaccharides including sulfated phosphorylated carboxymethylated acetylated and sulfonylated derivatives were prepared from a neutral polysaccharide (PD) extracted from Polygonatum cyrtonema Hua These compounds were characterized by FT-IR H-1 NMR and C-13 NMR spectroscopy Antiherpetic activities of natural and modified P cyrtonema polysaccharide against herpes simplex virus (HSV) Induced by cyclophosphamide (CP) were then evaluated on vero cells using cytopathic effect (CPE) inhibition assay The phorphorylated derivative (P-PD) and sulfated derivative (S-PD) exhibited significant inhibitory activity against HSV in comparison with the native ones especially P-PD presented better antiviral potency In addition the sulfonylated derivative (Ts-PD) was found to be as effective as PD and the acetylated derivative (Ac-PD) to be slightly less antiviral effective whereas carboxymethylated derivative (C-PD) was shown to be almost inactive The results indicated that the types of function groups appeared to be very important for the antiherpetic activity of polysaccharides (C) 2010 Elsevier Ltd All rights reserved