Isolation and characterization of eleven polymorphic tetranucleotide microsatellite loci for Quasipaa boulengeri (Anura: Dicroglossidae)

摘要

We isolated and characterized eleven polymorphic tetranucleotide microsatellite loci in the spiny-bellied frog (Quasipaa boulengeri). The loci were screened in 62 individuals from two natural populations. All loci were variable, with the number of alleles ranging from 8 to 26. The average observed and expected heterozygosity per locus ranged from 0.500 to 0.947 and from 0.735 to 0.932, respectively. Two loci were found to be significant departure from Hardy-Weinberg equilibrium and no significant linkage disequilibrium was detected after sequential Bonferroni corrections. These polymorphic loci will be useful for conservation and population genetics studies of this threated species.