3' non-templated 'A' addition by Taq DNA polymerase: An advantage in the construction of single and double mutants

摘要

The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3' non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferase