Cell growth and proliferative activities on titania nanotube arrays (TNA) have raised alerts on genotoxicity risk. Presentudtoxicogenomic approach focused on epithelial HT29 cells with TNA surface. Fledgling cell-TNA interaction has triggered G0/G1udcell cycle arrests and initiatesDNA damage surveillance checkpoint, which possibly indicated the cellular stress stimuli. A profoundudgene regulation was observed to be involved in cellular growth and survival signals such as p53 and AKT expressions. Interestingly,udthe activation of redox regulator pathways (antioxidant defense) was observed through the cascade interactions of GADD45, MYC,udCHECK1, and ATR genes.These mechanisms furnish to protect DNA during cellular division from an oxidative challenge, set inudmotion with XRRC5 and RAD50 genes for DNA damage and repair activities. The cell fate decision on TNA-nanoenvironmentudhas been reported to possibly regulate proliferative activities via expression of p27 and BCL2 tumor suppressor proteins, cogentudwith SKP2 and BCL2 oncogenic proteins suppression. Findings suggested that epithelial HT29 cells on the surface of TNA mayudhave a positive regulation via cell-homeostasis mechanisms: a careful circadian orchestration between cell proliferation, survival,udand death. This nanomolecular knowledge could be beneficial for advanced medical applications such as in nanomedicine andudnanotherapeutics.
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