In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum

摘要

Dendrobium sonia-28 adalah hibrid orkid yang penting dalam industri bunga di Malaysia kerana berkeupayaan berbunga berulang kali dan berbunga padat yang kini sedang menghadapi masalah pengeluaran yang serius akibat penyakit disebabkan kulat, terutamanya oleh Fusarium proliferatum. Untuk mengatasi masalah ini, salah satu strategi yang sedang diusahakan adalah dengan penghasilan orkid mutan baru. Mutagenesis secara in vitro dengan cara kultur turasan Fusarium proliferatum (CF), asid fusarik (FA) dan sinaran gama boleh digunakan untuk menghasilkan mutan yang lebih baik dari segi ekonomi. Dalam kajian ini, pemilihan jasad seperti protokom (JSP) yang bertoleransi terhadap F. proliferatum telah dijalankan dengan menilai kesan kepekatan CF (5-20%) dan FA (0.05-0.2 mM) dan pelbagai dos sinaran gama (10-200 Gy). Hasil kajian menunjukkan bahawa kadar hidup dan berat JSP berkadar songsang terhadap inokulasi dan sinaran dos. Selain itu, kadar kematian dan kekurangan berat JSP meningkat di kalangan JSP yang lebih kecil selepas rawatan CF dan FA. Keputusan menunjukkan bahawa ujian sensitiviti radio (LD50) bagi JSP lebih kurang pada 43 Gy.ududDendrobium sonia-28 is an important orchid hybrid in Malaysian flower industry for its flowering recurrence and dense inflorescences which currently facing serious production problems due to fungal diseases, especially caused by Fusarium proliferatum. To overcome this impediment, one of the strategies being pursued is by the production of new orchid mutants. In vitro mutagenesis by means of Fusarium proliferatum culture filtrate (CF), fusaric acid (FA) and gamma irradiation can be used to produce economically improved mutants. In this study, selection of Fusarium proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of different concentrations of CF (5-20%) and FA (0.05-0.2 mM) and various doses of gamma irradiation (10-200 Gy). Results showed that PLBs survival rate and weight were inversely related to the inoculation and irradiation doses. Additionally, PLBs death and weight reducing increased among smaller PLBs after CF and FA treatments. Results indicated that the radio sensitivity test (LD50) for the PLBs was approximately at 43 Gy.