Aktivitas Penghambatan Minyak Atsiri dan Ekstrak Kasar Biji Pala ud(Myristica fragrans Houtt dan Myristica fattua Houtt) terhadap udPertumbuhan Bakteri Xanthomonas campestris Oammel asal udTanaman Brokoli (Brassica oleracea var. italica)

摘要

The aims of this research were to study the inhibition activity of essential oil and crude extract of nutmeg seed ud(Myristica fragrans and M. fattua) on growth of Xanthomonas campestris which is pathogen to broccoli (Brassica oleracea udvar. italica), to find out the best concentration of those substances to prevent X. campestris and to compare the inhibition udactivity of both substances on growth of X. campestris. The essential oil of nutmeg seed (M. fragrans and M. fattua) was udobtained by Stahl distilation, while crude extract of the nutmeg seeds was extracted by metanol method. The method of udthis research was the disk diffusion method on nutrien agar. Paper disk was prestreaked on agar medium with X. udcampestris to be tested. Each petri disk was placed 5 paper disk dropped with the essential oil and the crude extract of udthe nutmeg seed in concentrations of 100%, 10%, 1% respectively, while for the comparison, the bactericide Agrept ud0,2% was used. The parameters measured were the inhibition zone from the essential oil and the crude extract of the udnutmeg seeds. The result of the analysis indicated that the essential oil of M. fragrans effectively inhibited X. campestris udstarting at concentration of 1%, the essential oil of M. fattua effectively inhibited X. campestris at concentration of 10% udand the crude extract of M. fragrans effectively inhibited X. campestris at concentration of 100%. The crude extract of M. udfattua was not effective on inhibiting the growth of X. campestris. ud udKey words: inhibition activity, nutmeg seed’s, Xanthomonas campestris.