Micro thermal analysis: A novel method of determining the extent of collagen cross-linking within a collagen construct- applications for tissue engineering

摘要

Increasingly complex designs of collagen scaffolds used in tissue engineering require more accurate and sensitive methods of testing. A new proposal to create cross-linked tunnels within a collagen scaffold for use in vascular and nerve studies will require a novel method of assessing the extent of cross-linking within the walls of the tube- to ensure maximal strength for matrix viability. To date this method has not yet been investigated.udAim: this study will investigate ways to differentiate between cross-linked and native collagen using melting characteristics found by Micro thermal analysis at point regions throughout the construct to give a detailed intra-matrix map.