Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

摘要

The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2udTC7 cells and a multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry.udIntracellular levels, effects on cadmium accumulation, distribution, and reference concentrationudranges of the following elements were determined: Na, Mg, Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd.udResults showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 toud10 lmol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, thisudaccumulation was more marked in cells exposed long-term to cadmium compared with controls, and thatudthis exposure resulted in a significant accumulation of copper and zinc but not of the other elementsudmeasured. Interactions of Cd with three elements: zinc, copper, and manganese were particularly studied.udExposed to 30 lmol/l of the element, manganese showed the highest inhibition and copper the lowest onudcadmium intracellular accumulation but Zn, Cu, and Mn behave differently in terms of their mutualudcompetition with Cd. Indeed, increasing cadmium in the culture medium resulted in a gradual and significantudincrease in the accumulation of zinc. There was a significant decrease in manganese from 5 lmoludCd/l exposure, and no variation was observed with copper.udAbbreviation: AAS – Atomic absorption spectrometry; CRM– Certified reference material; PBS – Phosphateudbuffered saline without calcium and magnesium; DMEM – Dubelcco’s modified Eagle’s medium.