In the present study, three techniques of extraction: hydrodistillation (HD), solvent extraction (conventional ‘Soxhlet’ technique) and an innovative technique, i.e., the supercritical fluid extraction (SFE), were applied to ground Tetraclinis articulata leaves and compared for extraction duration, extraction yield, and chemical composition of the extracts as well as their antioxidant activities. The extracts were analyzed by GC–FID and GC–MS. The antioxidant activity was measured using two methods: ABTS and DPPH. The yield obtained using HD, SFE, hexane and ethanol Soxhlet extractions were found to be 0.6, 1.6, 40.4 and 21.2–27.4 g/kg respectively. An original result of this study is that the best antioxidant activity was obtained with an SFE extract (41 mg/L). The SFE method offers some noteworthy advantages over traditional alternatives, such as shorter extraction times, low environmental impact, and a clean, non-thermally-degraded final product. Also, a good correlation between the phenolic contents and the antioxidant activity was observed with extracts obtained by SFE at 9 MPa.
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机译:在本研究中,将三种提取技术:加氢蒸馏(HD),溶剂提取(常规“索氏提取”技术)和一种创新技术(即超临界流体提取(SFE))应用于磨碎的Tetraclinis articulata叶片并进行比较以进行提取。提取物的持续时间,提取得率,化学成分及其抗氧化活性。通过GC-FID和GC-MS分析提取物。使用两种方法测量抗氧化活性:ABTS和DPPH。使用HD,SFE,己烷和乙醇索氏提取法获得的产量分别为0.6、1.6、40.4和21.2-27.4 g / kg。这项研究的原始结果是,使用SFE提取物(41 mg / L)获得了最佳的抗氧化活性。与传统的替代方法相比,SFE方法具有一些显着的优势,例如,提取时间更短,对环境的影响小以及清洁,非热降解的最终产品。另外,在9MPa下通过SFE获得的提取物观察到酚含量与抗氧化活性之间的良好相关性。
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