首页> 外文OA文献 >Self-adjuvanting vaccine against group A streptococcus: application of fibrillized peptide and immunostimulatory lipid as adjuvant
【2h】

Self-adjuvanting vaccine against group A streptococcus: application of fibrillized peptide and immunostimulatory lipid as adjuvant

机译:抗A组链球菌的自佐剂疫苗:原纤维化肽和免疫刺激脂质作为佐剂的应用

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Peptides are of great interest to be used as vaccine antigens due to their safety, ease of manufacturing and specificity in generating immune response. There have been massive discoveries of peptide antigens over the past decade. However, peptides alone are poorly immunogenic, which demand co-administration with strong adjuvant to enhance their immunogenicity. Recently, fibril-forming peptides such as Q11 and lipoamino acid-based carrier have been identified to induce substantial immune responses when covalently linked to peptide epitope. In this study, we have incorporated either Q11 or lipoamino acids to a peptide epitope (J14) derived from M protein of group A streptococcus to develop self-adjuvanting vaccines. J14, Q11 and lipoamino acids were also conjugated together in a single vaccine construct in an attempt to evaluate the synergy effect of combining multiple adjuvants. Physicochemical characterization demonstrated that the vaccine constructs folded differently and self-assembled into nanoparticles. Significantly, only vaccine constructs containing double copies of lipoamino acids (regardless in conjugation with Q11 or not) were capable to induce significant dendritic cells uptake and subsequent J14-specific antibody responses in non-sizes dependent manners. Q11 had minimal impact in enhancing the immunogenicity of J14 even when it was used in combination with lipoamino acids. These findings highlight the impact of lipoamino acids moiety as a promising immunostimulant carrier and its number of attachment to peptide epitope was found to have a profound effect on the vaccine immunogenicity.
机译:肽由于其安全性,易于制造和在产生免疫应答中的特异性而非常受关注用作疫苗抗原。在过去的十年中,已经有大量的肽抗原发现。然而,单独的肽免疫原性差,需要与强佐剂共同给药以增强其免疫原性。最近,已鉴定出当与肽表位共价连接时,诸如Q11和基于脂氨基酸的载体之类的原纤维形成肽可诱导大量的免疫反应。在这项研究中,我们已将Q11或脂氨基酸掺入A组链球菌M蛋白衍生的肽表位(J14),以开发自佐剂疫苗。 J14,Q11和脂氨基酸也被结合在单个疫苗构建物中,以试图评估多种佐剂结合的协同效应。物化特性表明,疫苗构建体折叠方式不同,并自组装成纳米颗粒。重要的是,仅含有双拷贝的脂氨基酸的疫苗构建体(无论是否与Q11结合)都能够以不依赖大小的方式诱导大量树突状细胞摄取和随后的J14特异性抗体反应。即使与脂氨基酸组合使用,Q11对增强J14的免疫原性的影响也很小。这些发现强调了脂氨基酸部分作为有希望的免疫刺激载体的影响,并且发现其与肽表位的连接数目对疫苗的免疫原性具有深远的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号