Experimental study of invasion and lymph node metastasis by orthotopic transplatation of human oral cancer cells tissue into tongue of nude mouse

摘要

ヒト口腔扁平上皮癌由来樹立細胞株3株 (HSC-2, HSC-3, HSC-4) を単離細胞状および組織片で, また手術時採取しヌードマウスで継代中の可移植株7株 (KOS-4, 5, 6, 7, 8, 9, 10) を組織片でそれぞれヌードマウス舌に同所性移植し, 局所浸潤および所属リンパ節転移について組織学的に検討した結果, 以下の所見を得た。1) いずれの移植材料ともヌードマウス舌への生着に成功し, 生着率は29～100%であった。2) 舌移植腫瘍は組織学的に周囲組織へ浸潤性に増殖していた。単離細胞移植と組織片移植とで組織像に明らかな差はなかった。3) HSC-2, 3, 4およびKOS-5, 7, 8で頸部リンパ節転移を認めた。転移率は13～80%であった。4) 以上の所見より, 本実験モデルは口腔癌の局所浸潤, 頚部リンパ節転移の in vivo の研究に有用と考えられた。 / Three human oral cancer cell lines (HSC-2, 3 and 4), both in the form of single cell suspension and tissue, and seven cancer tissues obtained by operation materials and maintained in nude mouse (KOS-4, 5, 6, 7, 8, 9 and 10) were transplanted orthotopically into the tongue of nude mouse. A histologic study conducted to examine local invasion and lymph node metastasis in this experimental model, and the results were as follows ; 1) Each material was transplantable into the tongue of nude mouse with the sucessful rate ranging from 29% to 100%. 2) Histologic examination of the transplanted tumor revealed invasive proliferation of cancer cells in the tongue of nude mouse. There were no apparent differences between the histologic features of single cells transplantation and those of cancer tissue transplantation. 3) Metastasis to the cervical lymph node was observed in animals into which HSC-2, HSC-3, HSC-4, KOS-5, KOS-7 and KOS-8 were transplanted. The rate of metastasis ranged from 13% to 80%. 4) These findings indicated that this experimental model is useful in in vivo studies of local invasion and lymph node metastasis of oral cancer.