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Contact X-ray microscopy of living cells by using LiF crystal as imaging detector

机译:以LiF晶体为成像检测器的活细胞接触X射线显微镜

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摘要

In this paper, the use of lithium fluoride (LiF) as imagingudradiation detector to analyse living cells by single-shot softudX-ray contact microscopy is presented. High resolved X-rayudimages on LiF of cyanobacterium Leptolyngbya VRUC135,udtwo unicellular microalgae of the genus Chlamydomonasudand mouse macrophage cells (line RAW 264.7) have beenudobtained utilizingX-ray radiation in the water window energyudrange from a laser plasma source. The used method is based onudloading of the samples, the cell suspension, in a special holderudwhere they are in close contactwith a LiF crystal solid-state Xrayudimaging detector. After exposure and sample removal, theudimages stored in LiF by the softX-ray contactmicroscopy techniqueudare read by an optical microscope in fluorescence mode.udThe clear image of the mucilaginous sheath the structureudof the filamentous Leptolyngbya and the visible nucleolus inudthe macrophage cells image, are noteworthiness results. Theudpeculiarities of the used X-ray radiation and of the LiF imaginguddetector allow obtaining images in absorption contrast revealingudthe internal structures of the investigated samples at highudspatial resolution. Moreover, thewidedynamicrangeof theLiFudimaging detector contributes to obtain high-quality images. Inudparticular, we demonstrate that this peculiar characteristic ofudLiF detector allows enhancing the contrast and reveal detailsudeven when they were obscured by a nonuniform stray light.
机译:本文介绍了氟化锂(LiF)作为成像放射检测器通过单次软 udX射线接触显微镜分析活细胞的方法。已使用水窗能量中的X射线辐射从激光获得的超范围获得了蓝细菌Lepttolyngbya VRUC135的LiF上的高分辨X射线 udimage,衣藻属的两个单细胞微藻 ud和小鼠巨噬细胞(线RAW 264.7)。等离子源。所使用的方法基于样品的装载,细胞悬液在特殊的固定器中的放置,其中样品与LiF晶体固态X射线数码成像检测器紧密接触。曝光并除去样品后,通过softX射线接触显微镜技术在LiF中存储的 udimage图像可以通过光学显微镜以荧光模式读取。 巨噬细胞图像,是值得注意的结果。所使用的X射线辐射和LiF成像 uddetector的特殊性允许在吸收对比度中获得图像,从而以高空间分辨率显示所研究样品的内部结构。此外,LiF Digmaging探测器的宽动态范围有助于获得高质量的图像。特别是,我们证明了 udLiF检测器的这一独特特性可以增强对比度并显示细节,即使它们被不均匀的杂散光遮盖也是如此。

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