For more than one century efforts has been made to obtain potato (Solanum tuberosum) cultivars resistant to late blight. However, introduced resistance has repeatedly been overcome by Phytophthora infestans (Mont) de Bary. Today late blight control is dependent on the frequent use of fungicides, but development of fungicide resistance and increasing fungicide restrictions by EU are of major concern. Methods with less fungicide requirement is therefore of crucial importance for a more environmentally sound and sustainable late blight control in the future.ududIn this study the potential of integrating BABA-induced resistance in existing late blight management with fungicides was investigated in field. The fungicide dose could be lowered with up to 25% when combined with BABA, without any decrease in late blight control or metabolic cost in terms of tuber yield. BABA was shown to directly activate basal defence responses and hormone signaling in potato. The BABA-induced hypersensitive-like lesions and major changes in the amino acid balance indicate that BABA induces resistance by stress imprinting. ududFurthermore the potential of using a biosurfactant, produced by Psuedomonas koreensis strain 2.74, to control late blight in greenhouse was demonstrated. The biosurfactant was shown to have a direct effect on zoospores and also to induce PR-1 accumulation in the apoplast of potato leaves. Future experiments will reveal if the biosurfactant induces other defence mechanisms in potato.ududThis study demonstrated how integration of different control methods could lead to unchanged or even improved late blight control despite the decrease in fungicide dose. ud
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机译:一个多世纪以来,人们一直在努力获得对晚疫病具有抗性的马铃薯(Solanum tuberosum)品种。但是,引入的抗性已被疫病疫霉(Monty de Bary)反复克服。如今,晚疫病的防治取决于杀真菌剂的频繁使用,但是欧盟对杀真菌剂的抗药性的发展和对杀真菌剂的限制日益增加是主要关注的问题。因此,对于未来的环境无害化和可持续性的控制,减少杀真菌剂需求的方法至关重要。 ud ud在这项研究中,我们对在田间进行的晚疫病防治中将BABA诱导的抗性与杀真菌剂整合的潜力进行了研究。与BABA结合使用时,杀菌剂的剂量最多可降低25%,而后期疫病控制或以块茎产量计的新陈代谢成本则不会降低。已证明BABA可直接激活马铃薯的基础防御反应和激素信号传导。 BABA诱导的过敏样病变和氨基酸平衡的重大变化表明BABA通过应激印迹诱导抗药性。 ud ud此外,还证明了使用朝鲜红假单胞菌2.74菌株生产的生物表面活性剂控制温室晚疫病的潜力。已证明该生物表面活性剂对游动孢子具有直接作用,并且还诱导马铃薯叶的质外体中PR-1积累。未来的实验将揭示生物表面活性剂是否会诱导马铃薯的其他防御机制。 ud ud这项研究表明,尽管杀真菌剂剂量减少了,但不同控制方法的整合如何能导致不变或改善后期疫病的防治。 ud
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